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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 1997/11 |
| 名称 | Heterogeneous expression of multiple putative patterning genes by single cells from the chick hindbrain |
| 区分 | その他 |
| 開催場所 | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS DEVELOPMENTAL BIOLOGY |
| 発表者・共同発表者等 | K Kato,DK ODowd,SE Fraser,MA Smith |
| 発表・展示等 | The metameric organization of the vertebrate hindbrain into rhombomeres appears to result from the patterned expression of several transcription factors and putative signaling molecules. We have applied a refined single-cell reverse transcription-polymerase chain reaction strategy to examine the molecular logic proposed to pattern the hindbrain at the single-cell level. This technique allows analysis of the concurrent expression of several genes within an individual cell at higher sensitivity than by in situ hybridization. Our results demonstrate that cells in rhombomere (r) 4 and r5 are heterogeneous in their expression of Hoxa-3, Hoxb-2, Sek-1, and Krox-20, suggesting that single cells are dynamically regulating their rhombomere-specific gene-expression profiles. Furthermore, the strong correlation between Sek-1 and Krox-20 expression at stage 12 was greatly diminished by stage 16, suggesting that the proposed interdependence of these two genes is present only at early stages of hindbrain development. (C) 1997 Academic Press. |
| DOI | 10.1006/dbio.1997.8717 |