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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 1999/07 |
| 名称 | Characterization of a novel monoclonal antibody that senses nitric oxide-dependent activation of soluble guanylate cyclase |
| 区分 | その他 |
| 開催場所 | ELSEVIER SCIENCE BV FEBS LETTERS |
| 発表者・共同発表者等 | S Tsuyama,E Yamazaki,T Tomita,H Ihara,S Takenaka,K Kato,S Kozaki |
| 発表・展示等 | Two monoclonal antibodies (mAbs) against bovine lung soluble guanylate cyclase (sGC) were prepared and characterized, mAb 3221 recognized both the alpha- and beta-subunits of sGC and had greater binding affinity to the enzyme in the presence of NO. mAb 28131 recognized only the beta-subunit and its affinity did not change with NO. Neither mAb cross-reacted with particulate GC. Cultured Purkinje cells from rats mere treated with S-nitroso-N-acetylpenicillamine, an NO donor, and examined by immunocytochemical methods. The immunoreactivity associated with mAb 3221 increased with the cGMP content in a crude extract of cerebellum and the NO2 generated in the culture medium increased. (C) 1999 Federation of European Biochemical Societies. |
| DOI | 10.1016/S0014-5793(99)00884-4 |