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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 2000/04 |
| 名称 | Neuropsin regulates an early phase of Schaffer-collateral long-term potentiation in the murine hippocampus |
| 区分 | その他 |
| 開催場所 | BLACKWELL SCIENCE LTD EUROPEAN JOURNAL OF NEUROSCIENCE |
| 発表者・共同発表者等 | S Komai,T Matsuyama,K Matsumoto,K Kato,M Kobayashi,K Imamura,S Yoshida,S Ugawa,S Shiosaka |
| 発表・展示等 | We found that neuropsin, an extracellular matrix serine protease, has a regulatory effect on Schaffer-collateral long-term potentiation (LTP) in the mouse hippocampus. Bath application of 1-170 nM recombinant neuropsin modulated early phase LTP in the Schaffer-collateral pathway with a 'bell-shape' dose-response curve. The maximum enhancing activity (134% of control LTP) was found at similar to 2.5 nM. Bath application of a neutralizing antibody against neuropsin in the hippocampal slice resulted in a marked inhibition of the tetanus-induced early phase of LTP. The in vivo continuous intraventricular infusion of an antisense oligonucleotide against neuropsin significantly reduced the amplitude of the tetanus-induced early phase of LTP in vitro. Neuropsin did not directly change the N-methyl D-aspartate (NMDA) current. Thus, neuropsin appears to act as a regulatory molecule in the early phase of LTP via its proteolytic function on extracellular matrix rather than affecting NMDA receptor-mediated calcium increase. |
| DOI | 10.1046/j.1460-9568.2000.00035.x |