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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 2001/03 |
| 名称 | Abnormalities of synapses and neurons in the hippocampus of neuropsin-deficient mice |
| 区分 | その他 |
| 開催場所 | ACADEMIC PRESS INC MOLECULAR AND CELLULAR NEUROSCIENCE |
| 発表者・共同発表者等 | A Hirata,S Yoshida,N Inoue,K Matsumoto-Miyai,A Ninomiya,M Taniguchi,T Matsuyama,K Kato,H Iizasa,Y Kataoka,N Yoshida,S Shiosaka |
| 発表・展示等 | In the present study,we produced null-mutant mice of neuropsin, an extracellular matrix serine protease, to examine the neural functions of this protein particularly in the hippocampus. Golgi-Cox impregnation and NissI-staining revealed morphological change of cell soma in the mutant mice compared to wild-type mice. However, Golgi-Cox impregnation revealed no apparent change in the dendritic arborization and spine density. Quantitative electronmicroscopic analysis revealed that number of asymmetrical synapses were significantly decreased in the stratum radiatum, the major terminal field of Schaffer-collaterals, whereas free boutons still holding synaptic vesicles but with no synaptic specialization were increased in number in the same microscopic fields. An increased number of parvalbumin-immunoreactive cells (known as fast spiking cells) in mutant was also observed. These results strongly suggest that neuropsin is involved in connectivity of a group of CA1 synapses and consequently in the hippocampal networking. |
| DOI | 10.1006/mcne.2000.0945 |