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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 2010/06 |
| 名称 | DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis |
| 区分 | その他 |
| 開催場所 | ELSEVIER IRELAND LTD JOURNAL OF REPRODUCTIVE IMMUNOLOGY |
| 発表者・共同発表者等 | Ken Takeshi Kusakabe,Hideaki Abe,Tomohiro Kondo,Keiko Kato,Toshiya Okada,Yoshinori Otsuki |
| 発表・展示等 | Gene expression profiling can be of benefit in identifying critical factors in the process of disease initiation and development. However, in endometriosis it has proven difficult to identify common genes between DNA microarray studies, presumably because of tissue homogeneity in lesions and diversity in the patients' conditions. We attempted DNA microarray analysis in a mouse model for endometriosis with stable lesions and a homogeneous genetic background. Data extracted from the mouse model was then evaluated in human tissues. Mice of the ddY strain underwent surgery to remove the left side of the uterine horn, and the uterine tissue was then minced into small segments and auto-transplanted onto the left peritoneum. After 8 weeks, most of the uterine grafts were enlarged and had regenerated lumens. Comparison of the intensity of mRNA expression between grafts and normal uteri showed that genes encoding immune regulators (e.g. CXCL10) and metabolic factors (e.g. calbindin D-28K) were highly up-regulated in the grafts. Strongly inhibited genes in the grafts included prostaglandin-related factors [e.g. prostaglandin E receptor 3 (subtype EP3) and prostaglandin I2 synthase]. Variation in some candidate factors detected in the mouse model was observed by immunohistochemical studies in human adenomyosis tissues. The gene list in the present study is available for re-evaluation of past studies and provides new candidate factors potentially involved in the pathogenesis of endometriosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved. |
| DOI | 10.1016/j.jri.2010.02.008 |