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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 期間 | 2010/08 |
| 名称 | Compensatory Renal Growth in Uninephrectomized Immature Rats: Proliferative Activity and Epidermal Growth Factor |
| 区分 | その他 |
| 開催場所 | JAPAN SOC VET SCI JOURNAL OF VETERINARY MEDICAL SCIENCE |
| 発表者・共同発表者等 | Toshiya Okada,Mika Omoto-Kitao,Masafumi Mukamoto,Jun Nakamura,Masaki Mino,Tomohiro Kondo,Ai Takeshita,Ken Takeshi Kusakabe,Keiko Kato |
| 発表・展示等 | Compensatory response to uninephrectomy in immature animals is stronger compared with that in adult ones and the response is due mainly to renal cell proliferation. The present study explored to show the growth pattern of the remaining kidney immediately after uninephrectomy in immature rats with special reference to proliferating activity and epidermal growth factor (EGF). Immunolocalizations of proliferating cell nuclear antigen (PCNA) and EGF in immature rat kidney were examined during the first three days after uninephrectomy. Semi-quantitative analysis of the expression of preproEGF mRNA was performed. One day after the operation, the PCNA positive cell ratios in the glomeruli and the proximal tubules were significantly higher in unilaterally nephrectomized (UNx) rats than in sham-operated (Sham) rats. In UNx and Sham rats, the proximal and distal tubular cells showed positive reactions to EGF antibody. The positive reaction of proximal tubules to EGF antibody was weaker in UNx than in Sham rats 1 day after the operation, while the degree of reactivity was not different between UNx and Sham rats 3 clays after the operation. The level of expression of preproEGF mRNA in the kidney was significantly lower in UNx than in Sham rats 1 day after the operation. These results indicate that unilateral nephrectomy in immature rats causes increased proliferative activity and decreased expression of EGF in the remaining kidney during the early period of compensatory renal growth. |
| DOI | 10.1292/jvms.09-0496 |