教員情報 - 加藤　啓子

カトウ　ケイコ KATO KEIKO 加藤　啓子所属京都産業大学生命科学部先端生命科学科職種教授
期間	1993/10
名称	USE OF THE HEMAGGLUTINATING VIRUS OF JAPAN (HVJ)-LIPOSOME METHOD FOR ANALYSIS OF INFILTRATING LYMPHOCYTES INDUCED BY HEPATITIS-B VIRUS GENE-EXPRESSION IN LIVER-TISSUE
区分	その他
開催場所	ELSEVIER SCIENCE BV BIOCHIMICA ET BIOPHYSICA ACTA
発表者・共同発表者等	K KATO,Y DOHI,Y YONEDA,K YAMAMURA,Y OKADA,M NAKANISHI
発表・展示等	We previously developed a method for introducing foreign genes into liver tissue using liposomes with incorporated hemagglutinating virus of Japan (HVJ, Sendai virus), and found that liver cells transfected with the E. coli beta-galactosidase gene or the gene for hepatitis B virus (HBV) surface protein (HBsAg) expressed these proteins in vivo. Here, we analyzed cellular reactions leading to hepatitis in the liver by expressing the genes of HBV in vivo. Lymphocytes were eluted directly from liver transfected with the HBsAg genes and shown to be cytotoxic only to cells expressing HBsAg in vitro. These lymphocytes were identified as cytotoxic T lymphocytes with the CD4- CD8+ phenotype. Transfer of these lymphocytes to transgenic mice with the whole HBV genome led to elevation of the serum glutamic-pyruvic transaminase (SGPT) level, indicating the induction of hepatitis due to the cytotoxic T lymphocytes in vivo. Similarly, direct transfer of the gene for the HBV secretory core protein (HBeAg) induced expression of HBeAg in hepatocytes and the appearance of antibody against HBeAg in the serum. However, using this system, we found that the lymphocytes infiltrating the transfected liver showed no cytotoxicity specific for HBeAg. These results indicate that expression of HBsAg, one of the components of virions, in animal liver induced hepatitis efficiently through generation of specific cytotoxic T lymphocytes (CTL) without any expression of the other viral components. This in vivo experimental system should be useful for evaluating how expression of a given gene induces cellular reactions and intrinsic functions in the living body.