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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Truncated form of tenascin-X, XB-S, interacts with mitotic motor kinesin Eg5 |
| 執筆形態 | その他 |
| 掲載誌名 | MOLECULAR AND CELLULAR BIOCHEMISTRY |
| 出版社・発行元 | SPRINGER |
| 巻・号・頁 | 320(1-2),pp.53-66 |
| 著者・共著者 | Toshiya Endo,Hiroyoshi Ariga,Ken-ichi Matsumoto |
| 概要 | XB-S is a protein with an amino-terminal-truncated form of tenascin-X (TNXB). However, the precise roles of XB-S in vivo are unknown. In this study, to determine the role of XB-S in vivo, we screened XB-S-binding proteins. FLAG-tagged XB-S was transiently introduced into 293T cells. Then its associated proteins were purified by immunoprecipitation using an anti-FLAG antibody and its components were identified by mass spectrometric analyses. Mitotic motor kinesin Eg5 was identified in the immunoprecipitates. XB-S and Eg5 proteins were co-localized in the cytoplasm in interphase and mitosis, but XB-S did not localize on mitotic spindle microtubules, on which Eg5 prominently localized in mitosis. As for Eg5 binding to XB-S, glutathione S-transferase-fused XB-S expressed in vitro directly bound to full-length Eg5 translated in reticulocyte lysate, and the XB-S-binding region was located in the motor domain of Eg5. Furthermore, during cell cycle progression XB-S showed a similar expression profile to that of Eg5. These results suggest possible involvement of XB-S in the function of Eg5. |
| DOI | 10.1007/s11010-008-9898-y |
| ISSN | 0300-8177 |
| PMID | 18679583 |