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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Roles of Tom70 in Import of Presequence-containing Mitochondrial Proteins |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
| 出版社・発行元 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 巻・号・頁 | 284(46),pp.31635-31646 |
| 著者・共著者 | Hayashi Yamamoto,Kenji Fukui,Hisashi Takahashi,Shingo Kitamura,Takuya Shiota,Kayoko Terao,Mayumi Uchida,Masatoshi Esaki,Shuh-ichi Nishikawa,Tohru Yoshihisa,Koji Yamano,Toshiya Endo |
| 概要 | Mitochondrial protein traffic requires precise recognition of the mitochondrial targeting signals by the import receptors on the mitochondrial surface including a general import receptor Tom20 and a receptor for presequence-less proteins, Tom70. Here we took a proteome-wide approach of mitochondrial protein import in vitro to find a set of presequence-containing precursor proteins for recognition by Tom70. The presequences of the Tom70-dependent precursor proteins were recognized by Tom20, whereas their mature parts exhibited Tom70-dependent import when attached to the presequence of Tom70-independent precursor proteins. The mature parts of the Tom70-dependent precursor proteins have the propensity to aggregate, and the presence of the receptor domain of Tom70 prevents their aggregate formation. Therefore Tom70 plays the role of a docking site for not only cytosolic chaperones but also aggregate-prone substrates to maintain their solubility for efficient transfer to downstream components of the mitochondrial import machineries. |
| DOI | 10.1074/jbc.M109.041756 |
| ISSN | 0021-9258 |