エンドウ トシヤ   ENDO TOSHIYA
  遠藤 斗志也
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   客員教授
言語種別 英語
発行・発表の年月 2011/01
形態種別 研究論文
査読 査読あり
標題 Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria
執筆形態 その他
掲載誌名 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版社・発行元 NATL ACAD SCIENCES
巻・号・頁 108(1),pp.91-96
著者・共著者 Hayashi Yamamoto,Nobuka Itoh,Shin Kawano,Yoh-ichi Yatsukawa,Takaki Momose,Tadashi Makio,Mayumi Matsunaga,Mihoko Yokota,Masatoshi Esaki,Toshihiro Shodai,Daisuke Kohda,Alyson E. Aiken Hobbs,Robert E. Jensen,Toshiya Endo
概要 Mitochondria import most of their resident proteins from the cytosol, and the import receptor Tom20 of the outer-membrane translocator TOM40 complex plays an essential role in specificity of mitochondrial protein import. Here we analyzed the effects of Tom20 binding on NMR spectra of a long mitochondrial presequence and found that it contains two distinct Tom20-binding elements. In vitro import and cross-linking experiments revealed that, although the N-terminal Tom20-binding element is essential for targeting to mitochondria, the C-terminal element increases efficiency of protein import in the step prior to translocation across the inner membrane. Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency.
DOI 10.1073/pnas.1014918108
ISSN 0027-8424