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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2011/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The intron of tRNA-Trp(CCA) is dispensable for growth and translation of Saccharomyces cerevisiae |
| 執筆形態 | その他 |
| 掲載誌名 | RNA-A PUBLICATION OF THE RNA SOCIETY |
| 出版社・発行元 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| 巻・号・頁 | 17(9),pp.1760-1769 |
| 著者・共著者 | Shunsuke Mori,Takuya Kajita,Toshiya Endo,Tohru Yoshihisa |
| 概要 | A part of eukaryotic tRNA genes harbor an intron at one nucleotide 3' to the anticodon, so that removal of the intron is an essential processing step for tRNA maturation. While some tRNA introns have important roles in modification of certain nucleotides, essentiality of the tRNA intron in eukaryotes has not been tested extensively. This is partly because most of the eukaryotic genomes have multiple genes encoding an isoacceptor tRNA. Here, we examined whether the intron of tRNA-Trp(CCA) genes, six copies of which are scattered on the genome of yeast, Saccharomyces cerevisiae, is essential for growth or translation of the yeast in vivo. We devised a procedure to remove all of the tRNA introns from the yeast genome iteratively with marker cassettes containing both positive and negative markers. Using this procedure, we removed all the introns from the six tRNA-Trp(CCA) genes, and found that the intronless strain grew normally and expressed tRNA-Trp(CCA) in an amount similar to that of the wild-type genes. Neither incorporation of S-35-labeled amino acids into a TCA-insoluble fraction nor the major protein pattern on SDS-PAGE/2D gel were affected by complete removal of the intron, while expression levels of some proteins were marginally affected. Therefore, the tRNA-Trp(CCA) intron is dispensable for growth and bulk translation of the yeast. This raises the possibility that some mechanism other than selective pressure from translational efficiency maintains the tRNA intron on the yeast genome. |
| DOI | 10.1261/rna.2851411 |
| ISSN | 1355-8382 |