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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2013/05 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Tam41 Is a CDP-Diacylglycerol Synthase Required for Cardiolipin Biosynthesis in Mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | CELL METABOLISM |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 17(5),pp.709-718 |
| 著者・共著者 | Yasushi Tamura,Yoshihiro Harada,Shuh-ichi Nishikawa,Koji Yamano,Megumi Kamiya,Takuya Shiota,Takuya Kuroda,Osamu Kuge,Hiromi Sesaki,Kenichiro Imai,Kentaro Tomii,Toshiya Endo |
| 概要 | CDP-diacylglycerol (CDP-DAG) is central to the phospholipid biosynthesis pathways in cells. A prevailing view is that only one CDP-DAG synthase named Cds1 is present in both the endoplasmic reticulum (ER) and mitochondrial inner membrane (IM) and mediates generation of CDP-DAG from phosphatidic acid (PA) and CTP. However, we demonstrate here by using yeast Saccharomyces cerevisiae as a model organism that Cds1 resides in the ER but not in mitochondria, and that Tam41, a highly conserved mitochondrial maintenance protein, directly catalyzes the formation of CDP-DAG from PA in the mitochondrial IM. We also find that inositol depletion by overexpressing an arrestin-related protein Art5 partially restores the defects of cell growth and CL synthesis in the absence of Tam41. The present findings unveil the missing step of the cardiolipin synthesis pathway in mitochondria as well as the flexibile regulation of phospholipid biosynthesis to respond to compromised CDP-DAG synthesis in mitochondria. |
| DOI | 10.1016/j.cmet.2013.03.018 |
| ISSN | 1550-4131/1932-7420 |