エンドウ トシヤ   ENDO TOSHIYA
  遠藤 斗志也
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   客員教授
言語種別 英語
発行・発表の年月 2020/12
形態種別 研究論文
標題 Organelle membrane-specific chemical labeling and dynamic imaging in living cells.
執筆形態 その他
掲載誌名 Nature chemical biology
掲載区分国外
巻・号・頁 16(12),pp.1361-1367
著者・共著者 Tomonori Tamura,Alma Fujisawa,Masaki Tsuchiya,Yuying Shen,Kohjiro Nagao,Shin Kawano,Yasushi Tamura,Toshiya Endo,Masato Umeda,Itaru Hamachi
概要 Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking.
DOI 10.1038/s41589-020-00651-z
PMID 32958953