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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Characterization of the targeting signal in mitochondrial beta-barrel proteins |
| 執筆形態 | その他 |
| 掲載誌名 | NATURE COMMUNICATIONS |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 7,pp.12036 |
| 著者・共著者 | Tobias Jores,Anna Klinger,Lucia E. Gross,Shin Kawano,Nadine Flinner,Elke Duchardt-Ferner,Jens Woehnert,Hubert Kalbacher,Toshiya Endo,Enrico Schleiff,Doron Rapaport |
| 概要 | Mitochondrial beta-barrel proteins are synthesized on cytosolic ribosomes and must be specifically targeted to the organelle before their integration into the mitochondrial outer membrane. The signal that assures such precise targeting and its recognition by the organelle remained obscure. In the present study we show that a specialized beta-hairpin motif is this long searched for signal. We demonstrate that a synthetic beta-hairpin peptide competes with the import of mitochondrial beta-barrel proteins and that proteins harbouring a beta-hairpin peptide fused to passenger domains are targeted to mitochondria. Furthermore, a beta-hairpin motif from mitochondrial proteins targets chloroplast beta-barrel proteins to mitochondria. The mitochondrial targeting depends on the hydrophobicity of the beta-hairpin motif. Finally, this motif interacts with the mitochondrial import receptor Tom20. Collectively, we reveal that beta-barrel proteins are targeted to mitochondria by a dedicated beta-hairpin element, and this motif is recognized at the organelle surface by the outer membrane translocase. |
| DOI | 10.1038/ncomms12036 |
| ISSN | 2041-1723 |
| PMID | 27345737 |