エンドウ トシヤ   ENDO TOSHIYA
  遠藤 斗志也
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   客員教授
言語種別 英語
発行・発表の年月 2016/07
形態種別 研究論文
査読 査読あり
標題 A phospholipid transfer function of ER-mitochondria encounter structure revealed in vitro
執筆形態 その他
掲載誌名 SCIENTIFIC REPORTS
出版社・発行元 NATURE PUBLISHING GROUP
巻・号・頁 6,pp.30777
著者・共著者 Rieko Kojima,Toshiya Endo,Yasushi Tamura
概要 As phospholipids are synthesized mainly in the endoplasmic reticulum ( ER) and mitochondrial inner membranes, how cells properly distribute specific phospholipids to diverse cellular membranes is a crucial problem for maintenance of organelle-specific phospholipid compositions. Although the ER-mitochondria encounter structure (ERMES) was proposed to facilitate phospholipid transfer between the ER and mitochondria, such a role of ERMES is still controversial and awaits experimental demonstration. Here we developed a novel in vitro assay system with isolated yeast membrane fractions to monitor phospholipid exchange between the ER and mitochondria. With this system, we found that phospholipid transport between the ER and mitochondria relies on membrane intactness, but not energy sources such as ATP, GTP or the membrane potential across the mitochondrial inner membrane. We further found that lack of the ERMES component impairs the phosphatidylserine transport from the ER to mitochondria, but not the phosphatidylethanolamine transport from mitochondria to the ER. This in vitro assay system thus offers a powerful tool to analyze the non-vesicular phospholipid transport between the ER and mitochondria.
DOI 10.1038/srep30777
ISSN 2045-2322
PMID 27469264