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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Expression of mammalian mitochondrial F-1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2 |
| 執筆形態 | その他 |
| 掲載誌名 | FEBS OPEN BIO |
| 出版社・発行元 | WILEY |
| 巻・号・頁 | 6(12),pp.1267-1272 |
| 著者・共著者 | Toshiharu Suzuki,Naoya Iida,Junko Suzuki,Yasunori Watanabe,Toshiya Endo,Toru Hisabori,Masasuke Yoshida |
| 概要 | F1-ATPase (F1) is a multisubunit water-soluble domain of FoF1-ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F1 assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F1 in Escherichia coli cells. Intactness of the function and conformation of the E. coli-expressed bovine F1 was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F1. |
| DOI | 10.1002/2211-5463.12143 |
| ISSN | 2211-5463 |
| PMID | 28203526 |