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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/03/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structure-function insights into direct lipid transfer between membranes by Mmm 1-Mdm 12 of ERMES |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Cell Biology |
| 出版社・発行元 | Rockefeller University Press |
| 巻・号・頁 | 217(3),pp.959-974 |
| 著者・共著者 | Shin Kawano,Yasushi Tamura,Rieko Kojima,Siqin Bala,Eri Asai,Agnès H. Michel,Benoît Kornmann,Isabelle Riezman,Howard Riezman,Yoshitake Sakae,Yuko Okamoto,Toshiya Endo |
| 概要 | The endoplasmic reticulum (ER)-mitochondrial encounter structure (ERMES) physically links the membranes of the ER and mitochondria in yeast. Although the ER and mitochondria cooperate to synthesize glycerophospholipids, whether ERMES directly facilitates the lipid exchange between the two organelles remains controversial. Here, we compared the x-ray structures of an ERMES subunit Mdm 12 from Kluyveromyces lactis with that of Mdm 12 from Saccharomyces cerevisiae and found that both Mdm 12 proteins possess a hydrophobic pocket for phospholipid binding. However in vitro lipid transfer assays showed that Mdm 12 alone or an Mmm1 (another ERMES subunit) fusion protein exhibited only a weak lipid transfer activity between liposomes. In contrast, Mdm 12 in a complex with Mmm1 mediated efficient lipid transfer between liposomes. Mutations in Mmm1 or Mdm 12 impaired the lipid transfer activities of the Mdm 12-Mmm1 complex and furthermore caused defective phosphatidylserine transport from the ER to mitochondrial membranes via ERMES in vitro. Therefore, the Mmm 1-Mdm 12 complex functions as a minimal unit that mediates lipid transfer between membranes. |
| DOI | 10.1083/jcb.201704119 |
| ISSN | 1540-8140 |
| PMID | 29279306 |