エンドウ トシヤ   ENDO TOSHIYA
  遠藤 斗志也
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   客員教授
言語種別 英語
発行・発表の年月 1994/04
形態種別 研究論文
査読 査読あり
標題 YGE1P, A EUKARYOTIC GRP-E HOMOLOG, IS LOCALIZED IN THE MITOCHONDRIAL MATRIX AND INTERACTS WITH MITOCHONDRIAL HSP70
執筆形態 その他
掲載誌名 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版社・発行元 ACADEMIC PRESS INC ELSEVIER SCIENCE
巻・号・頁 200(1),pp.435-442
著者・共著者 M NAKAI,Y KATO,E IKEDA,A TOHE,T ENDO
概要 Yeast Yge1p, the gene product of YGE1, is a eukaryotic GrpE homolog found recently (Ikeda et al., 1994). We have revealed here that Yge1p is a soluble protein in the mitochondrial matrix. Depletion of Yge1p in yeast cells resulted in accumulation of the precursor of F-1-ATPase a subunit in vivo, suggesting that Yge1p is involved in protein import into mitochondria. Overexpression of Yge1p in the temperature-sensitive mutant strains of mitochondrial hsp70, Ssc1p, caused hypersensitivity to temperature for cell growth, suggesting a genetic interaction between the YGE1 and SSC1 genes. A physical interaction between Yge1p and Ssc1p was directly demonstrated by co-immunoprecipitation of Ssc1p by the anti-Yge1p antibodies. We propose that Yge1p functions in cooperation with Ssc1p in a similar manner as bacterial GrpE with DnaK. (C) 1994 Academic Press, Inc.
ISSN 0006-291X/1090-2104