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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1994/12 |
| 形態種別 | その他 |
| 査読 | 査読あり |
| 標題 | IDENTIFICATION OF THE SECA PROTEIN HOMOLOG IN PEA-CHLOROPLASTS AND ITS POSSIBLE INVOLVEMENT IN THYLAKOIDAL PROTEIN-TRANSPORT |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
| 出版社・発行元 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 巻・号・頁 | 269(50),pp.31338-31341 |
| 著者・共著者 | M NAKAI,A GOTO,T NOHARA,D SUGITA,T ENDO |
| 概要 | Recently, we identified the SecA and SecY proteins in the cyanobacterium Synechococcus PCC7942, Antibodies raised against cyanobacterial SecA specifically reacted with a 110-kDa protein of pea chloroplasts, suggesting the presence of SecA in higher plant chloroplasts. Apart of the pea secA cDNA was polymerase chain reaction-amplified with degenerated oligonucleotide primers and with pea cDNA as a template. The deduced amino acid sequence shows 62% identity with cyanobacterial SecA and 52% identity with Escherichia coli Seek Antibodies raised against the pea SecA fragment, which was expressed in E. coli cells hom the obtained polymerase chain reaction-amplified cDNA, reacted with the 110-kDa chloroplast protein; the 110-kDa protein was mainly found in the stroma but partly in the thylakoid membrane. The anti-pea SecA IgG inhibited the in vitro import of the 33-kDa protein of the oxygen-evolving complex, but not of the 23-kDa protein of the oxygen-evolving complex, into thylakoids. These results suggest that SecA facilitates transport of a subset of thylakoid lumenal proteins including the 33-kDa protein into thylakoids, We propose that a bacterial-type Sec protein-dependent transport system operates for protein transport into thylakoids in higher plant chloroplasts. |
| ISSN | 0021-9258/1083-351X |