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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes |
| 執筆形態 | その他 |
| 掲載誌名 | CELL |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 111(4),pp.519-528 |
| 著者・共著者 | H Yamamoto,M Esaki,T Kanamori,Y Tamura,S Nishikawa,T Endo |
| 概要 | Based on the results of site-specific photocrosslinking of translocation intermediates, we have identified Tim50, a component of the yeast TIM23 import machinery, which mediates translocation of presequence-containing proteins across the mitochondrial inner membrane. Tim50 is anchored to the inner mitochondrial membrane, exposing the C-terminal domain to the intermembrane space. Tim50 interacts with the N-terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti-Tim50 antibodies block the protein translocation across the inner membrane. A translocation intermediate accumulated at the TOM complex is crosslinked to Tim50. We suggest that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the TIM23 complex. |
| ISSN | 0092-8674 |