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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Tom40 protein import channel binds to non-native proteins and prevents their aggregation |
| 執筆形態 | その他 |
| 掲載誌名 | NATURE STRUCTURAL BIOLOGY |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 10(12),pp.988-994 |
| 著者・共著者 | M Esaki,T Kanamori,S Nishikawa,Shin, I,PG Schultz,T Endo |
| 概要 | Mitochondria contain the translocator of the outer mitochondrial membrane (TOM) for protein entry into the organelle, and its subunit Tom40 forms a protein-conducting channel. Here we report the role of Tom40 in protein translocation across the membrane. The site-specific photocrosslinking experiment revealed that translocating unfolded or loosely folded precursor segments of up to 90 residues can be associated with Tom40. Purified Tom40 bound to non-native proteins and suppressed their aggregation when they are prone to aggregate. A denatured protein bound to the Tom40 channel blocked the protein import into mitochondria. These results indicate that, in contrast to the nonstick tunnel of the ribosome for polypeptide exit, the Tom40 channel offers an optimized environment to translocating non-native precursor proteins by preventing their aggregation. |
| DOI | 10.1038/nsb1008 |
| ISSN | 1072-8368 |