エンドウ トシヤ   ENDO TOSHIYA
  遠藤 斗志也
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   客員教授
言語種別 英語
発行・発表の年月 2005/03
形態種別 研究論文
査読 査読あり
標題 The phosphate carrier has an ability to be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria
執筆形態 その他
掲載誌名 JOURNAL OF BIOLOGICAL CHEMISTRY
出版社・発行元 AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
巻・号・頁 280(11),pp.10011-10017
著者・共著者 K Yamano,D Ishikawa,M Esaki,T Endo
概要 Most mitochondrial proteins are synthesized in the cytosol, imported into mitochondria via the TOM40 ( translocase of the mitochondrial outer membrane 40) complex, and follow several distinct sorting pathways to reach their destination submitochondrial compartments. Phosphate carrier (PiC) is an inner membrane protein with 6 transmembrane segments (TM1-TM6) and requires, after translocation across the outer membrane, the Tim9-Tim10 complex and the TIM22 complex to be inserted into the inner membrane. Here we analyzed an in vitro import of fusion proteins between various PiC segments and mouse dihydrofolate reductase. The fusion protein without TM1 and TM2 was translocated across the outer membrane but was not inserted into the inner membrane. The fusion proteins without TM1-TM4 were not inserted into the inner membrane but instead translocated across the inner membrane. Functional defects of Tim50 of the TIM23 complex caused either by depletion of the protein or the addition of anti-Tim50 antibodies blocked translocation of the fusion proteins without TM1-TM4 across the inner membrane, suggesting that lack of TM1-TM4 led to switch of its sorting pathway from the TIM22 pathway to the TIM23 pathway. PiC thus appears to have a latent signal for sorting to the TIM23 pathway, which is exposed by reduced interactions with the Tim9-Tim10 complex and maintenance of the import competence.
DOI 10.1074/jbc.M413264200
ISSN 0021-9258
PMID 15644337