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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1997/11 |
| 形態種別 | その他 |
| 標題 | Heterogeneous expression of multiple putative patterning genes by single cells from the chick hindbrain |
| 執筆形態 | その他 |
| 掲載誌名 | DEVELOPMENTAL BIOLOGY |
| 出版社・発行元 | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS |
| 巻・号・頁 | 191(2),pp.259-269 |
| 著者・共著者 | K Kato,DK ODowd,SE Fraser,MA Smith |
| 概要 | The metameric organization of the vertebrate hindbrain into rhombomeres appears to result from the patterned expression of several transcription factors and putative signaling molecules. We have applied a refined single-cell reverse transcription-polymerase chain reaction strategy to examine the molecular logic proposed to pattern the hindbrain at the single-cell level. This technique allows analysis of the concurrent expression of several genes within an individual cell at higher sensitivity than by in situ hybridization. Our results demonstrate that cells in rhombomere (r) 4 and r5 are heterogeneous in their expression of Hoxa-3, Hoxb-2, Sek-1, and Krox-20, suggesting that single cells are dynamically regulating their rhombomere-specific gene-expression profiles. Furthermore, the strong correlation between Sek-1 and Krox-20 expression at stage 12 was greatly diminished by stage 16, suggesting that the proposed interdependence of these two genes is present only at early stages of hindbrain development. (C) 1997 Academic Press. |
| DOI | 10.1006/dbio.1997.8717 |
| ISSN | 0012-1606 |