カトウ ケイコ   KATO KEIKO
  加藤 啓子
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2001/05
形態種別 その他
標題 Dendritic aberrations in the hippocampal granular layer and the amygdalohippocampal area following kindled-seizures
執筆形態 その他
掲載誌名 BRAIN RESEARCH
出版社・発行元 ELSEVIER SCIENCE BV
巻・号・頁 901(1-2),pp.281-295
著者・共著者 K Kato,T Masa,Y Tawara,K Kobayashi,T Oka,A Okabe,S Shiosaka
概要 Amygdaloid kindling is a model of human temporal lobe epilepsy, in which excitability in limbic structures is permanently enhanced by repeated stimulations. We report here dendritic aberrations occurring in mice following kindled-seizures. Adult mice received a biphasic square wave pulse [495 +/- 25.5 (S.E.M.) muA 60 Hz, 200 mus duration. for 2 s] unilaterally in the basolateral amygdaloid complex once a day and mice with electrophysiologically and behaviorally verified seizures were used in the experiments. The hippocampus and amygdaloid complex contralateral to the lesions were observed by immunofluorescence histochemistry with a somatodendritic marker, microtubule-associated protein 2 (MAP2). showing that kindled-seizures caused hypertrophy of proximal dendrites in the granule cells of the dentate gyrus and in neurons of the amygdalohippocampal area. To further characterize the morphological changes of the dendrites, electron micrographic analysis was performed on the contralateral side. (1) In the granular layer of the dentate gyrus and the amygdalohippocampal area, kindled-seizures generated an increase in the number of dendrites containing polymerized microtubules and width of dendritic profiles showing the increase was in the range 0.2-3.0 and 0.2-1.4 mum. respectively. (2) In the granular layer, bundles between dendrites separated by the puncta adhaerentia increased. (3) In the granular layer, the seizure-induced dendritic aberration was more severe in the rostral than the caudal region. These results suggested that growth of dendrites with enriched-stable microtubules is part of the structural plasticity in response: to seizure activity in specific areas of the adult brain. (C) 2001 Elsevier Science B.V. All rights reserved.
DOI 10.1016/S0006-8993(01)02299-5
ISSN 0006-8993