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カトウ ケイコ
KATO KEIKO
加藤 啓子 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/01 |
| 形態種別 | その他 |
| 標題 | Region-specific and epileptogenic-dependent expression of six subtypes of alpha 2,3-sialyltransferase in the adult mouse brain |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF NEUROCHEMISTRY |
| 出版社・発行元 | BLACKWELL PUBLISHING LTD |
| 巻・号・頁 | 84(1),pp.53-66 |
| 著者・共著者 | H Matsuhashi,Y Horii,K Kato |
| 概要 | Sialylated glycoconjugates play important roles in various biological functions. The structures are also observed in brains and it has been proposed that sialylation may affect neural plasticity. To clarify the effects of sialylation in the brain, particular neurons that exhibit sialylation should first be determined. Using in situ hybridization, we performed systematic surveys of the localization of mRNAs encoding the six alpha2,3-sialyltransferases (ST3Gal I-VI) in the adult mouse brain with or without physiological stimulation. First, striking region-specific patterns of expression were observed: While ST3Gal II, III, and V mRNAs were in neuronal cells throughout the brain, ST3Gal I, IV, and VI mRNAs were in restricted brain regions. Next, to assess whether the expression of the six mRNAs can be regulated, we examined the effect of kindling epileptogenesis on the six mRNA levels. Of the six subtypes, upregulation in the ST3Gal IV level in the thalamus was most pronounced; the number of ST3Gal IV-expressing neurons in the anterior thalamic nuclei increased from 2% to 21% in a time-dependent manner during epileptogenesis. Western blot analysis evaluated the increase of the end-products in the thalamus. These findings provide a molecular basis to clarify when and where sialylated glycoconjugates function accompanied by neural plasticity. |
| DOI | 10.1046/j.1471-4159.2003.01257.x |
| ISSN | 0022-3042 |