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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2015/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The Ingenious Structure of Central Rotor Apparatus in VoV1; Key for Both Complex Disassembly and Energy Coupling between V-1 and V-o |
| 執筆形態 | その他 |
| 掲載誌名 | PLOS ONE |
| 出版社・発行元 | PUBLIC LIBRARY SCIENCE |
| 巻・号・頁 | 10(3),pp.e0119602 |
| 著者・共著者 | Atsuko Nakanishi,Jun-ichi Kishikawa,Masatada Tamakoshi,Ken Yokoyama |
| 概要 | Vacuolar type rotary H+-ATPases (VoV1) couple ATP synthesis/hydrolysis by V-1 with proton translocation by V-o via rotation of a central rotor apparatus composed of the V-1-DF rotor shaft, a socket-like V-o-C (eukaryotic V-o-d) and the hydrophobic rotor ring. Reconstitution experiments using subcomplexes revealed a weak binding affinity of V-1-DF to V-o-C despite the fact that torque needs to be transmitted between V-1-DF and V-o-C for the tight energy coupling between V-1 and V-o. Mutation of a short helix at the tip of V-1-DF caused intramolecular uncoupling of VoV1, suggesting that proper fitting of the short helix of V-1-D into the socket of V-o-C is required for tight energy coupling between V-1 and V-o. To account for the apparently contradictory properties of the interaction between V-1-DF and V-o-C (weak binding affinity but strict requirement for torque transmission), we propose a model in which the relationship between V-1-DF and V-o-C corresponds to that between a slotted screwdriver and a head of slotted screw. This model is consistent with our previous result in which the central rotor apparatus is not the major factor for the association of V-1 with V-o (Kishikawa and Yokoyama, J Biol Chem. 2012 24597-24603). |
| DOI | 10.1371/journal.pone.0119602 |
| ISSN | 1932-6203 |
| PMID | 25756791 |