ヨコヤマ ケン   YOKOYAMA KEN
  横山 謙
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 1990/10/01
形態種別 研究論文
査読 査読あり
標題 Electron microscopy of the reconstituted complexes of the F1-ATPase with various subunit constitution revealed the location of the γ subunit in the central cavity of the molecule
執筆形態 その他
掲載誌名 FEBS Letters
巻・号・頁 271(1-2),pp.111-115
著者・共著者 Yuichiro Fujiyama,Ken Yokoyama,Masasuke Yoshida,Takeyuki Wakabayashi
概要 The subunit structure of the F1-ATPase from the thermophilic bacterium PS3 was probed by comparing electron microscopic images of the subunit complexes reconstituted to contain different subunit compositions. The following structural features were observed. (1) The α3β3 complex has a hexagonal, apparently symmetrical arrangement of masses with a central cavity. (2) The projections of the α3β3δ complexes are similar to those of the α3β3 complexes. (3) In contrast, the α3β3λ complex has an additional mass in the centre which is similar to that found in the native enzyme (α3β3γδε{lunate}). From these observations, it is concluded that the central mass in the F1-ATPase is comprised mostly of the γ subunit. © 1990.
DOI 10.1016/0014-5793(90)80384-U
ISSN 0014-5793
PMID 2146152