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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Evidence for rotation of V-1-ATPase |
| 執筆形態 | その他 |
| 掲載誌名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| 出版社・発行元 | NATL ACAD SCIENCES |
| 巻・号・頁 | 100(5),pp.2312-2315 |
| 著者・共著者 | H Imamura,M Nakano,H Noji,E Muneyuki,S Ohkuma,M Yoshida,K Yokoyama |
| 概要 | VoV1-ATPase is responsible for acidification of eukaryotic intracellular compartments and ATP synthesis of Archaea and some eubacteria. From the similarity to FoF1-ATP synthase, VoV1-ATPase has been assumed to be a rotary motor, but to date there are no experimental data to support this. Here we visualized the rotation of single molecules of V-1-ATPase, a catalytic subcomplex of VoV1-ATPase. V-1-ATPase from Thermus thermophilus was immobilized onto a glass surface, and a bead was attached to the D or IF subunit through the biotin-streptavidin linkage. In both cases we observed ATP-dependent rotations of beads, the direction of which was always counterclockwise viewed from the membrane side. Given that three ATP molecules are hydrolyzed per one revolution, rates of rotation agree consistently with rates of ATP hydrolysis at saturating ATP concentrations. This study provides experimental evidence that VoV1-ATPase is a rotary motor and that both D and F subunits constitute a rotor shaft. |
| DOI | 10.1073/pnas.0436796100 |
| ISSN | 0027-8424 |
| PMID | 12598655 |