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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization of the chaperonin GroEL-GroES complex from Thermus thermophilus HB8 |
| 執筆形態 | その他 |
| 掲載誌名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY |
| 出版社・発行元 | BLACKWELL MUNKSGAARD |
| 巻・号・頁 | 59(Pt 9),pp.1632-1634 |
| 著者・共著者 | T Shimamura,A Koike-Takeshita,K Yokoyama,M Yoshida,H Taguchi,S Iwata |
| 概要 | The chaperonin GroEL-GroES (GroEL/ES) complex from a thermophilic eubacteria, Thermus thermophilus HB8, has been purified and crystallized. The GroEL/ES complex is known to be composed of 14 identical GroEL subunits (58 kDa) and seven identical GroES subunits (11 kDa). The GroEL/ES complex crystals belong to the triclinic space group P1, with unit-cell parameters a=140.4, b=156.4, c =273.1 Angstrom, alpha=82.9, beta=85.4, gamma=68.5degrees. The crystal asymmetric unit contains two molecules (MW=885 kDa). One data set to 3.0 Angstrom resolution, with 383 652 independent observations (89.3% complete) and an R-merge of 0.08, has been collected from a single crystal. A molecular-replacement solution was obtained using the structure of the GroEL/ES complex from Escherichia coli as a search model. |
| DOI | 10.1107/S0907444903013660 |
| ISSN | 0907-4449 |
| PMID | 12925795 |