ヨコヤマ ケン   YOKOYAMA KEN
  横山 謙
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2008/07
形態種別 研究論文
査読 査読あり
標題 Molecular mechanism of energy conservation in polysulfide respiration
執筆形態 その他
掲載誌名 Nature Structural and Molecular Biology
巻・号・頁 15(7),pp.730-737
著者・共著者 Mika Jormakka,Ken Yokoyama,Takahiro Yano,Masatada Tamakoshi,Satoru Akimoto,Tatsuro Shimamura,Paul Curmi,So Iwata
概要 Bacterial polysulfide reductase (PsrABC) is an integral membrane protein complex responsible for quinone-coupled reduction of polysulfide, a process important in extreme environments such as deep-sea vents and hot springs. We determined the structure of polysulfide reductase from Thermus thermophilus at 2.4-Å resolution, revealing how the PsrA subunit recognizes and reduces its unique polyanionic substrate. The integral membrane subunit PsrC was characterized using the natural substrate menaquinone-7 and inhibitors, providing a comprehensive representation of a quinone binding site and revealing the presence of a water-filled cavity connecting the quinone binding site on the periplasmic side to the cytoplasm. These results suggest that polysulfide reductase could be a key energy-conserving enzyme of the T. thermophilus respiratory chain, using polysulfide as the terminal electron acceptor and pumping protons across the membrane via a previously unknown mechanism. © 2008 Nature Publishing Group.
DOI 10.1038/nsmb.1434
ISSN 1545-9993
PMID 18536726