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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization and preliminary X-ray crystallographic studies of monoacylglycerol lipase of the moderately thermophilic Bacillus sp H-257 |
| 執筆形態 | その他 |
| 掲載誌名 | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY |
| 出版社・発行元 | BLACKWELL MUNKSGAARD |
| 巻・号・頁 | 58,pp.1232-1233 |
| 担当区分 | 最終著者,責任著者 |
| 著者・共著者 | K Yoneda,T Nishimura,N Katunuma,S Imamura,K Nitta,H Tsuge |
| 概要 | Thermostable monoacylglycerol lipase (MGLP; EC 3.1.1.23) from the moderately thermophilic Bacillus sp. H-257 has a unique substrate specificity. It hydrolyzes monoacylglycerols but does not hydrolyze di- or triacylglycerols. Crystals of the enzyme were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and benzamidine as an additive. The orthorhombic crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 43.53, b = 100.82, c = 108.17 Angstrom. The crystals diffract to at least 2.3 Angstrom resolution and a native data set has been collected to 2.6 Angstrom resolution on a CCD detector using synchrotron radiation. |
| DOI | 10.1107/S0907444902006169 |
| ISSN | 0907-4449 |