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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Chondroitin sulfate proteoglycan is a potent enhancer in the processing of procathepsin L |
| 執筆形態 | その他 |
| 掲載誌名 | BIOLOGICAL CHEMISTRY |
| 出版社・発行元 | WALTER DE GRUYTER & CO |
| 巻・号・頁 | 383(12),pp.1925-1929 |
| 著者・共著者 | M Kihara,H Kakegawa,Y Matano,E Murata,H Tsuge,H Kido,N Katunuma |
| 概要 | The acceleration effect of chondroitin-4-sulfate(CS-) proteoglycan on the processing of procathepsin L in vitro was investigated using enzyme purified from the culture medium of MLC cells. Procathepsin L was slightly processed even when it was incubated without CS-proteoglycan for 60 min in 50 mM acetate buffer, pH 5.5, and trace amounts of the 31 kDa mature form and 35-38 kDa intermediates of cathepsin L were formed. On the other hand, in the presence of CS-proteoglycan, procathepsin L was completely converted to the mature form within the same 60 minute time period. Moreover, Z-Phe-Arg-MCA hydrolyzing activity was increased significantly by the incubation with CS-proteoglycan, while no considerable increase in the activity was observed during the incubation without CS-proteoglycan. Since the specific cathepsin L inhibitor, CLIK-195, inhibited the processing of procathepsin L accelerated by CS-proteoglycan, the trace amount of cathepsin L activity may participate in the processing. These results suggest that CS-proteoglycan may play a role in accelerating the processing of procathepsin L as an endogenous enhancer in the extracellular environment in vivo. |
| DOI | 10.1515/BC.2002.216 |
| ISSN | 1431-6730 |