ツゲ ヒデアキ   TSUGE HIDEAKI
  津下 英明
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2004/04
形態種別 研究論文
査読 査読あり
標題 Crystallization and preliminary X-ray diffraction analysis of the hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum
執筆形態 その他
掲載誌名 ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
出版社・発行元 BLACKWELL MUNKSGAARD
巻・号・頁 60(60(4)),pp.715-717
担当区分 最終著者,責任著者
著者・共著者 MW Bhuiya,H Sakuraba,K Yoneda,T Ohshima,T Imagawa,N Katunuma,H Tsuge
概要 NAD-dependent glutamate dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum was crystallized in the apo- and holoenzyme forms. Crystals were obtained using 2-propanol and polyethylene glycol MME 550 as precipitants for the apoenzyme and holoenzyme, respectively. The apoenzyme crystals belong to the trigonal space group P3(1)21 or its enantiomorph P3(2)21. The asymmetric unit contains three subunits; the values of the Matthews coef'cient (V-M) and the solvent content are 2.9 Angstrom(3) Da(-1) and 57%, respectively. A native data set was collected to a highest resolution limit of 4.0 Angstrom on an in-house X-ray source using a rotating-anode generator (overall R-sym of 12.3% and completeness of 97%). The holoenzyme crystals belong to the orthorhombic space group P2(1)2(1)2(1); the asymmetric unit contains one hexamer, giving a VM of 2.79 Angstrom(3) Da(-1) and a solvent content of 55%. Native and derivative data sets were collected. The crystals diffract to a maximum resolution of 2.8 Angstrom on the KEK-NW12 beamline at the Photon Factory and gave a data set with an overall R-sym of 7.9% and a completeness of 91%. Attempts are being made to solve the structure by the SIRAS method.
DOI 10.1107/S0907444904001854
ISSN 0907-4449