ツゲ ヒデアキ   TSUGE HIDEAKI
  津下 英明
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2004/11
形態種別 研究論文
査読 査読あり
標題 Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I
執筆形態 その他
掲載誌名 ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
出版社・発行元 BLACKWELL MUNKSGAARD
巻・号・頁 60(60(11)),pp.2006-2008
担当区分 最終著者,責任著者
著者・共著者 T Imagawa,H Nakayama,N Katunuma,H Sakuraba,T Ohshima,T Itoh,Y Sako,N Nomura,H Tsuge
概要 Two crystal forms, rhombohedral and hexagonal, of a homing endonuclease from Thermoproteus sp. IC-061 (I-Tsp0611) were obtained by the hanging-drop and sitting-drop method, respectively. The hexagonal crystals belong to space group P6(3)22, with unit-cell parameters a = b = 111.4, c = 97.6 Angstrom, and diffract to 3.2 Angstrom resolution on beamline BL44 at SPring-8 (Harima, Japan). The rhombohedral crystals belong to space group R32, with unit-cell parameters a = b = 95.4, c = 192.9 Angstrom, and diffract to 2.7 Angstrom resolution using a Cu Kalpha rotating-anode generator with an R-AXIS VII detector. The crystal asymmetric unit contained one protein molecule and the solvent contents of the two crystal forms were estimated to be 68.3 and 67.6% by volume, respectively.
DOI 10.1107/S0907444904020232
ISSN 0907-4449