ツゲ ヒデアキ   TSUGE HIDEAKI
  津下 英明
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2012
形態種別 研究論文
査読 査読あり
標題 Role of sphingomyelinase in infectious diseases caused by Bacillus cereus
執筆形態 その他
掲載誌名 PLoS ONE
出版社・発行元 PUBLIC LIBRARY SCIENCE
巻・号・頁 7(6)
著者・共著者 Oda, M.,Hashimoto, M.,Takahashi, M.,Ohmae, Y.,Seike, S.,Kato, R.,Fujita, A.,Tsuge, H.,Nagahama, M.,Ochi, S.,Sasahara, T.,Hayashi, S.,Hirai, Y.,Sakurai, J.
概要 Bacillus cereus (B. cereus) is a pathogen in opportunistic infections. Here we show that Bacillus cereus sphingomyelinase (Bc-SMase) is a virulence factor for septicemia. Clinical isolates produced large amounts of Bc-SMase, grew in vivo, and caused death among mice, but ATCC strains isolated from soil did not. A transformant of the ATCC strain carrying a recombinant plasmid containing the Bc-SMase gene grew in vivo, but that with the gene for E53A, which has little enzymatic activity, did not. Administration of an anti-Bc-SMase antibody and immunization against Bc-SMase prevented death caused by the clinical isolates, showing that Bc-SMase plays an important role in the diseases caused by B. cereus. Treatment of mouse macrophages with Bc-SMase resulted in a reduction in the generation of H2O2 and phagocytosis of macrophages induced by peptidoglycan (PGN), but no effect on the release of TNF-alpha and little release of LDH under our experimental conditions. Confocal laser microscopy showed that the treatment of mouse macrophages with Bc-SMase resulted in the formation of ceramide-rich domains. A photobleaching analysis suggested that the cells treated with Bc-SMase exhibited a reduction in membrane fluidity. The results suggest that Bc-SMase is essential for the hydrolysis of SM in membranes, leading to a reduction in phagocytosis.
DOI 10.1371/journal.pone.0038054
ISSN 1932-6203
Put Code(ORCID) 6110960
PermalinkURL http://www.scopus.com/inward/record.url?eid=2-s2.0-84862017218&partnerID=MN8TOARS