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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1991 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | F-ACTIN BUNDLING PROTEIN FROM PHYSARUM-POLYCEPHALUM - PURIFICATION AND ITS CAPACITY FOR CO-BUNDLING OF ACTIN-FILAMENTS AND MICROTUBULES |
| 執筆形態 | その他 |
| 掲載誌名 | CELL MOTILITY AND THE CYTOSKELETON |
| 出版社・発行元 | WILEY-LISS |
| 巻・号・頁 | 19(4),pp.244-254 |
| 著者・共著者 | N ITANO,S HATANO |
| 概要 | An F-actin bundling protein was isolated and purified from plasmodium of Physarum polycephalum. The F-actin bundling protein in Physarum extract was passed through a DEAE-cellulose column. After the protein in the fraction was treated with 6 M urea, it was purified by gel filtration on Sephacryl S-300 HR followed by chromatography on CM-Toyopearl (cation exchange) in the presence of 6 M urea. The purified protein gave a single band on SDS-PAGE, and the molecular weight was estimated to be 52,000. This F-actin bundling protein is referred to as the 52 kDa protein.
Interestingly, the 52 kDa protein also induced bundling of microtubules. The formation of F-actin and microtubule bundles was Ca2+-insensitive, but depended on the salt concentration. Each bundle formed at NaCl concentrations less than 0.1 M. The 52 kDa protein cross-reacted with monoclonal antibody raised against a HeLa 55 kDa protein (an F-actin bundling protein from HeLa cells) (Yamashiro-Matsumura and Matsumura: J. Biol. Chem. 260:5087-5097, 1985). When the 52 kDa protein was added to a mixture of actin filaments and microtubules, co-bundles composed of both filaments formed. This is the first reported example in which an F-actin bundling protein induced co-bundling of actin filaments and microtubules. |
| ISSN | 0886-1544 |