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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1998/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The gene structure and promoter sequence of mouse hyaluronan synthase 1 (mHAS1) |
| 執筆形態 | その他 |
| 掲載誌名 | BIOCHEMICAL JOURNAL |
| 出版社・発行元 | PORTLAND PRESS |
| 巻・号・頁 | 330,pp.1223-1227 |
| 著者・共著者 | Y Yamada,N Itano,M Zako,M Yoshida,P Lenas,A Niimi,M Ueda,K Kimata |
| 概要 | The structure and organization of mouse hyaluronan synthase 1 gene, HAS1 were determined by direct sequencing of lambda phage clones carrying the entire gene and by application of the long and accurate (LA)-PCR method to amplify regions encompassing the exon-intron boundaries and all of the exons. This gene spans about 11 kb of genomic DNA and consists of 5 exons and 4 introns. A similarity in the exon-intron organization was found between the genes of mouse HAS1 and Xenopus laevis DG42 which was recently identified as Xenopus hyaluronan synthase. The transcription initiation site was determined by rapid amplification of the cDNA ends (5'-RACE). Position +1 is located 55 nucleotides upstream of the ATG initiation codon. The promoter region of the HASI gene has no typical TATA box, but contains a CCAAT box located 190 nucleotides upstream of the transcription initiation site. Further analysis of 1.4 kb of the 5' flanking region revealed several potential binding motifs for transcription factors. This information about the gene structure may be useful for further studies on the promoter activity. |
| ISSN | 0264-6021 |