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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1999/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Metabolic activities of partially degenerated hypertrophic chondrocytes: gene expression of hyaluronan synthases |
| 執筆形態 | その他 |
| 掲載誌名 | CELL AND TISSUE RESEARCH |
| 出版社・発行元 | SPRINGER VERLAG |
| 巻・号・頁 | 298(2),pp.317-325 |
| 著者・共著者 | Y Takada,H Sakiyama,K Kuriiwa,R Masuda,N Inoue,K Nakagawa,N Itano,T Saito,T Yamada,K Kimata |
| 概要 | Ultrastructural aspects of hypertrophic chondrocytes in hamster and mouse epiphysial cartilage were examined in relation to their metabolic activities. With the hypertrophic change, cytoplasmic vacuolization proceeded leaving the partially intact endoplasmic reticulum (ER). In the hypertrophic cells, cytoplasmic hyaluronan was stained with the biotinylated hyaluronan-binding region (b-HABR) of aggrecan, and mRNAs of hyaluronan synthase (Has I, Has 2 and Has 3) were detected by in situ hybridization. When the epiphysial cartilage was cultured in the presence of S-35, H-3-GlcNAc, H-3-proline or C-14-palmitic acid, vacuolated late hypertrophic chondrocytes were labeled with these radioactive precursors. The evidence indicates that late hypertrophic chondrocytes are metabolically active, which appears to be essential for the enlargement of chondrocytes. |
| ISSN | 0302-766X |