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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2004/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Differential regulation by IL-1 beta and EGF of expression of three different hyaluronan synthases in oral mucosal epithelial cells and fibroblasts and dermal fibroblasts: Quantitative analysis using real-time RT-PCR |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF INVESTIGATIVE DERMATOLOGY |
| 出版社・発行元 | BLACKWELL PUBLISHING INC |
| 巻・号・頁 | 122(3),pp.631-639 |
| 著者・共著者 | Y Yamada,N Itano,K Hata,M Ueda,K Kimata |
| 概要 | Using "real-time RT-PCR", we assessed the expression of three different hyaluronan synthase genes, HAS1, HAS2, and HAS3, by measuring their mRNA amounts in cultured human oral mucosal epithelial (COME) cells, oral mucosal fibroblasts, and dermal fibroblasts, and investigated the effects of interleukin-1beta (IL-1beta) and epidermal growth factor (EGF). When COME cells were treated with IL-1beta or EGF, early and marked increases and subsequent rapid decreases were observed for all HAS genes and, moreover, actual changes in hyaluronan synthesis subsequently occurred. The effects of IL-1beta stimulation were concentration-dependent and the maximal response to the EGF stimulation was observed at a low concentration (0.1 ng per mL). When two different types of fibroblasts were treated with IL-1beta or EGF, increased expression with different degrees and rates of three different HAS genes and subsequent increased synthesis of hyaluronan were also observed. In addition, HAS1 gene expression was not detectable in the mucosal fibroblasts, while weak HAS3 gene expression was detected in the dermal fibroblasts. Taken together, it is likely that the regulation of the expression of the three different HAS genes is different between oral mucosa and skin, which may be of significance for elucidating some of the differences between these tissues in wound healing. |
| DOI | 10.1111/j.0022-202X.2004.22332.x |
| ISSN | 0022-202X |