イタノ ナオキ   ITANO NAOKI
  板野 直樹
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2007/02
形態種別 研究論文
査読 査読あり
標題 Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells
執筆形態 その他
掲載誌名 MEDICAL MYCOLOGY
出版社・発行元 TAYLOR & FRANCIS LTD
巻・号・頁 45(1),pp.73-79
著者・共著者 Akiyoshi Sakai,Sumio Akifusa,Naoki Itano,Koji Kimata,Taro Kawamura,Takeyoshi Koseki,Tadamichi Takehara,Tatsuji Nishihara
概要 Candida albicans is both a commensal and a pathogen in the oral mucosa. Previous studies have indicated that epithelial cell-associated carbohydrate moiety can inhibit C albicans growth. In the present study, the mechanisms by which epithelial cells inhibit Candida growth were studied by examining the effect of hyaluronan (HA). A coculture of C albicans and KB cells or COS-7 cells inhibited in vitro growth of the fungus by 50-87% at an effector-to-target (E:T) ratio of 80:1. Removing extracellular HA by hyaluronidase caused a significant decrease in the anti-Candida activity of the cells. In addition anti-Candida activity was observed at 1 mu g/ml HA (2000 kDa). The antifungal activity of extracellular HA was further studied by transiently transfecting COS-7 cells with human HSA1, HSA2, or HSA3 in order to produce high levels of extracellular HA. All of the transfectants inhibited C albicans growth in vitro by 51-65% compared to 38% inhibition by the vector control (P < 0.05). These results suggest that the anti-Candida activity of epithelial-cells is mediated by extracellular HA.
DOI 10.1080/13693780601039607
ISSN 1369-3786