イタノ ナオキ   ITANO NAOKI
  板野 直樹
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2007/07
形態種別 研究論文
査読 査読あり
標題 Versican, a major hyaluronan-binding component in the dermis, loses its hyaluronan-binding ability in solar elastosis
執筆形態 その他
掲載誌名 JOURNAL OF INVESTIGATIVE DERMATOLOGY
出版社・発行元 NATURE PUBLISHING GROUP
巻・号・頁 127(7),pp.1657-1663
著者・共著者 Keiko Hasegawa,Masahiko Yoneda,Hiroko Kuwabara,Osamu Miyaishi,Naoki Itano,Akiko Ohno,Masahiro Zako,Zenzo Isogai
概要 Versican interacts with hyaluronan (HA) at its N-terminus and with fibrillin-1 at its C terminus. As versican in the dermis connects microfibrils to the HA-rich matrix for viscoelasticity, dermal diseases may involve destruction of these complexes. A recombinant versican protein, rVN, covering the HA binding region (HABR) of human versican and a polyclonal antibody, 6084, against rVN were prepared and characterized. Blotting analyses of skin extracts with 6084 and biotin-conjugated HA revealed that versican was a major HA-binding component in the dermis. Matrix metalloprotease-12, which is expressed in areas of solar elastosis, degraded versican and abrogated its HA-binding ability. Immunohistochemical analyses revealed that the elastic materials in solar elastosis lesions were negative for 6084, but positive for 21311, an antibody recognizing the C-terminus of versican, indicating loss of the HABR in the aggregated elastic fibers. This loss of the HA-binding ability of versican followed by HA exclusion may be responsible for the pathological and phenotypical changes observed in solar elastosis.
DOI 10.1038/sj.jid.5700754
ISSN 0022-202X