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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation |
| 執筆形態 | その他 |
| 掲載誌名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS |
| 出版社・発行元 | ELSEVIER SCIENCE INC |
| 巻・号・頁 | 471(1),pp.85-93 |
| 著者・共著者 | Ikuko Kakizaki,Naoki Itano,Koji Kimata,Katsumi Hanada,Atsushi Kon,Masanori Yamaguchi,Teruno Takahashi,Keiichi Takagaki |
| 概要 | Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and both were up-regulated after a single irradiation with moderate UVB but hyaluronidases was unchanged. Increased accumulation of hyaluronan in the culture medium mirrored the UVB-induced increase in the mRNA levels of HAS3 and HAS2. Unexpectedly, hyaluronan derived from UVB-irradiated and non-irradiated cells had identical size distribution. Increased expression of KGF and IL-1 beta was detected just prior to the increase of HAS3 and HAS2 mRNAs after UVB irradiation. Antibody-neutralization study revealed that KGF and/or IL-1 beta were at least involved in the up-regulation of HAS3 and HAS2 expressions. UVB-irradiated cells may enhance hyaluronan production to maintain homeostasis through up-regulation of HAS3 and HAS2 genes via cytokine response mechanism. (c) 2007 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.abb.2007.12.004 |
| ISSN | 0003-9861 |