イタノ ナオキ   ITANO NAOKI
  板野 直樹
   所属   京都産業大学  生命科学部 先端生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2009
形態種別 研究論文
査読 査読あり
標題 A Splice Variant of ASC Regulates IL-1 beta Release and Aggregates Differently from Intact ASC
執筆形態 その他
掲載誌名 MEDIATORS OF INFLAMMATION
出版社・発行元 HINDAWI PUBLISHING CORPORATION
巻・号・頁 2009,pp.287387
著者・共著者 Kazuhiko Matsushita,Michiko Takeoka,Junji Sagara,Naoki Itano,Yuka Kurose,Akihiro Nakamura,Shun'ichiro Taniguchi
概要 The apoptosis-associated speck-like protein containing a caspase recruit domain (ASC) is involved in apoptosis and innate immunity and is a major adaptor molecule responsible for procaspase-1 activation. ASC mRNA is encoded by three exons: exons 1 and 3 encode a pyrin domain (PYD) and caspase recruit domain (CARD), respectively, and exon 2 encodes a proline and glycine-rich (PGR) domain. Here, we identified a variant ASC protein (vASC) lacking the PGR domain that was smaller than full length ASC (fASC) derived from fully transcribed mRNA and searched for differences in biochemical and biological nature. Both fASC and vASC were found to activate procaspase-1 to a similar degree, but the efficiency of IL-1 beta excretion was significantly higher for vASC. There was also a marked structural difference observed in the fibrous aggregates formed by fASC and vASC. These results suggest that although the PGR domain is dispensable for procaspase-1 activation, it plays an important role in the regulation of the molecular structure and activity of ASC. Copyright (C) 2009 Kazuhiko Matsushita et al.
DOI 10.1155/2009/287387
ISSN 0962-9351