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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Involvement of CD44 in mast cell proliferation during terminal differentiation |
| 執筆形態 | その他 |
| 掲載誌名 | LABORATORY INVESTIGATION |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 89(4),pp.446-455 |
| 著者・共著者 | Hirotsugu Takano,Shunsuke Nakazawa,Naritoshi Shirata,Shigero Tamba,Kazuyuki Furuta,Sohken Tsuchiya,Kazushi Morimoto,Naoki Itano,Atsushi Irie,Atsushi Ichikawa,Koji Kimata,Kazuhisa Nakayama,Yukihiko Sugimoto,Satoshi Tanaka |
| 概要 | By using the recently established culture system that reproduces the terminal differentiation process of connective tissue-type mast cells, we found significant transcriptional induction of CD44. As CD44 is a primary receptor for hyaluronan (HA), which is one of the major extracellular matrix components, we investigated the role of CD44 in cutaneous mast cells. When co-cultured with fibroblasts, mouse bone marrow-derived cultured mast cells (BMMCs) were found to form clusters in an HA-dependent manner. As compared with BMMCs derived from the wild-type mice, those from the CD44(-/-) mice exhibited impaired growth during the co-cultured period. Furthermore, in the peritoneal cavities and ear tissues, mature mast cells were fewer in number in the CD44(-/-) mice than in the wild-type mice. We investigated roles of CD44 in mast cell proliferation by reconstituting BMMCs into the tissues of mast cell-deficient, Kit(W)/Kit(W-v) mice, and found that the number of metachromatic cells upon acidic toluidine blue staining in the tissues transplanted with CD44(-/-) BMMCs was not significantly changed for 10 weeks, whereas that in the tissues transplanted with the CD44(+/+) BMMCs was significantly increased. These results suggest that CD44 plays a crucial role in the regulation of the cutaneous mast cell number. |
| DOI | 10.1038/labinvest.2008.159 |
| ISSN | 0023-6837 |