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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2010/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Calponin h1-S175T Point Mutation Enhances Resistance to Actin Cytoskeleton Perturbation in Human Cancer Cells |
| 執筆形態 | その他 |
| 掲載誌名 | ANTICANCER RESEARCH |
| 出版社・発行元 | INT INST ANTICANCER RESEARCH |
| 巻・号・頁 | 30(4),pp.1071-1078 |
| 著者・共著者 | Akemi Sugaya,Michiko Takeoka,Naoki Itano,Ayako Taguchi,Takashi Ehara,Shun'ichiro Taniguchi |
| 概要 | To investigate Calponin h1 (CNh1) regions responsible for suppressing cancer cell motility, the interaction between CNh1 and actin in HeLa cells was examined. First, it was observed that the actin binding of CNh1 depends on the calponin repeat 1 (CNR 1), region more than the actin binding site region. Next, point mutantions were generated at S175 and/or T184 in CNR1, substrates of protein kinase C, and it was observed by cellular immunostaining that the actin binding of CNh1 depends on 175th amino acid. Furthermore, the point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. This result indicates that S175T may be an effective target for new cancer treatments. |
| ISSN | 0250-7005 |