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テラチ トオル
TERACHI TORU
寺地 徹 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1999/06 |
| 形態種別 | その他 |
| 標題 | Identification of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by bulked segregant analysis |
| 執筆形態 | その他 |
| 掲載誌名 | BREEDING SCIENCE |
| 出版社・発行元 | JAPANESE SOC BREEDING |
| 巻・号・頁 | 49(2),pp.115-121 |
| 著者・共著者 | S Murayama,H Yamagishi,T Terachi |
| 概要 | DNA markers linked to a fertility restorer (Rf) gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) were screened by bulked segregant analysis using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and high-resolution agarose gel electrophoresis. Ten male-fertile and 10 male-sterile plants arbitrarily selected from the F-2 segregating population made by the selfing of an F-1 plant between 'MS-Gensuke' (a male-sterile line possessing Ogura cytoplasm) and 'Comet' (a restorer having a nuclear Rf gene), were used as the materials for bulked DNA samples. Using 10 out of 260 arbitrary primers tested, RAPD fragments specific to the bulked DNA samples from male-fertile F-2 plants were identified. Further linkage analysis performed with 89 individuals in the F-2 segregating population showed that the seven RAPD markers were linked to the Rf gene. RAPD markers distributed on both sides of the Rf gene, and the closest marker, OPH11(410) was positioned 1.2 cM from the Rf gene. The three closest RAPD markers to the Rf gene were converted to SCAR markers by molecular cloning and nucleotide sequencing. This allowed identification of more reliable DNA markers linked to the Rf gene than the RAPD fragments. |
| DOI | 10.1270/jsbbs.49.115 |
| ISSN | 1344-7610/1347-3735 |