テラチ トオル   TERACHI TORU
  寺地 徹
   所属   京都産業大学  生命科学部 産業生命科学科
   職種   教授
言語種別 英語
発行・発表の年月 2001/12
形態種別 その他
標題 Sequence analysis on the mitochondrial orfB locus in normal and Ogura male-sterile cytoplasms from wild and cultivated radishes
執筆形態 その他
掲載誌名 CURRENT GENETICS
出版社・発行元 SPRINGER-VERLAG
巻・号・頁 40(4),pp.276-281
著者・共著者 T Terachi,K Yamaguchi,H Yamagishi
概要 In order to gain a better understanding of the origin and evolution of Ogura male-sterile cytoplasm in radish, sequence analysis was conducted for the mitochondrial locus, orfB, using wild and cultivated radishes. The nucleotide sequence of the entire coding and flanking regions of orfB (similar to1 kb) was determined for six radishes with normal and Ogura cytoplasm and they were classified into three types (types 1-3). The sequence of the 5' flanking region of orfB was further analyzed in 40 additional plants. Irrespective of the category of radish, plants with Ogura male-sterile cytoplasm contained only the type 1 sequence, whereas plants with normal cytoplasm had either type 2 or type 3. The results suggested that the mutational event, which led to the association of orfB with the male-sterile gene orf138, had occurred only once in the history of radishes.
DOI 10.1007/s00294-001-0256-9
ISSN 0172-8083