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テラチ トオル
TERACHI TORU
寺地 徹 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003 |
| 形態種別 | その他 |
| 標題 | Identification of a sequence-tagged site (STS) marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish (Raphanus sativus L.) by non-radioactive AFLP analysis |
| 執筆形態 | その他 |
| 掲載誌名 | EUPHYTICA |
| 出版社・発行元 | KLUWER ACADEMIC PUBL |
| 巻・号・頁 | 129(1),pp.61-68 |
| 著者・共著者 | S Murayama,T Habuchi,H Yamagishi,T Terachi |
| 概要 | To identify DNA markers linked to a fertility restorer ( Rf) gene for Ogura cytoplasmic male sterility in radish ( Raphanus sativus L.), a non- radioactive, amplified fragment length polymorphism( AFLP) analysis was performed on bulked DNA samples from male- sterile and male- fertile radishes. Ten male- fertile and 10 male- sterile plants selected arbitrarily from an F-2 population made by selfing of F-1 plant from a cross between a male- sterile ('MS-Gensuke') plant and a restorer ('Comet') plant were used as material. Using 32 AFLP primer pairs, one AFLP fragment ( AFLP190) which is specific to the bulked DNA samples from male- fertile F-2 plants was identified. AFLP190 was characterized by molecular cloning and nucleotide sequencing, and was converted to a sequence-tagged site ( STS) marker, STS190. A linkage analysis performed in 126 individuals of two independent F-2 populations showed tight linkage of STS190 to the Rf gene. The rate of recombination between the marker and Rf was estimated to be less than 1%, making STS190 1.2 cM from the gene. |
| DOI | 10.1023/A:1021513331079 |
| ISSN | 0014-2336 |