|
ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 1995 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Characterization of a cis-Golgi matrix protein, GM130 |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Cell Biology |
| 出版社・発行元 | ROCKEFELLER UNIV PRESS |
| 巻・号・頁 | 131(6 II),pp.1715-1726 |
| 著者・共著者 | Nakamura, N.,Rabouille, C.,Watson, R.,Nilsson, T.,Hui, N.,Slusarewicz, P.,Kreis, T.E.,Warren, G. |
| 概要 | Antisera raised to a detergent- and salt-resistant matrix fraction from rat liver Golgi stacks were used to screen an expression library from rat liver cDNA. A full-length clone was obtained encoding a protein of 130 kD (termed GM130), the COOH-terminal domain of which was highly homologous to a Golgi human auto-antigen, golgin-95 (Fritzler et al., 1993). Biochemical data showed that GM130 is a peripheral cytoplasmic protein that is tightly bound to Golgi membranes and part of a larger oligomeric complex. Predictions from the protein sequence suggest that GM130 is an extended rod-like protein with coiled-coil domains, Immunofluorescence microscopy showed partial over-lap with medial- and trans-Golgi markers but almost complete overlap with the cis-Golgi network (CGN) marker, syntaxin5, Immunoelectron microscopy confirmed this location showing that most of the GM130 was located in the CGN and in one or two cisternae on the cis-side of the Golgi stack. GM130 was not re-distributed to the ER in the presence of brefeldin A but maintained its overlap with syntaxin5 and a partial overlap with the ER-Golgi intermediate compartment marker, p53, Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure. |
| DOI | 10.1083/jcb.131.6.1715 |
| ISSN | 0021-9525/1540-8140 |
| Put Code(ORCID) | 19809428 |