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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2017/04/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Functional characterisation of the YIPF protein family in mammalian cells |
| 執筆形態 | その他 |
| 掲載誌名 | Histochemistry and Cell Biology |
| 出版社・発行元 | Springer Verlag |
| 巻・号・頁 | 147(4),pp.439-451 |
| 著者・共著者 | Tilen Kranjc,Eugene Dempsey,Gerard Cagney,Nobuhiro Nakamura,Denis C. Shields,Jeremy C. Simpson |
| 概要 | In this study, we carry out a systematic characterisation of the YIPF family of proteins with respect to their subcellular localisation profile, membrane topology and functional effects on the endomembrane system. YIPF proteins primarily localise to the Golgi complex and can be grouped into trans-Golgi-localising YIPFs (YIPF1 and YIPF2) and cis-Golgi-localising YIPFs (YIPF3, YIPF4 and YIPF5), with YIPF6 and YIPF7 showing a broader profile being distributed throughout the Golgi stack. YIPF proteins have a long soluble N-terminal region, which is orientated towards the cytosol, followed by 5 closely stacked transmembrane domains, and a C terminus, orientated towards the lumen of the Golgi. The significance of YIPF proteins for the maintenance of the morphology of the Golgi was tested by RNA interference, revealing a number of specific morphological changes to this organelle on their depletion. We propose a role for this family of proteins in regulating membrane dynamics in the endomembrane system. |
| DOI | 10.1007/s00418-016-1527-3 |
| ISSN | 1432-119X |
| Put Code(ORCID) | 77737908 |